Synthesis of virus components

The nucleus of the infected cell harbors an integrated provirus. Unspliced viral transcripts are used to synthesize Gag and Gag-Pro-Pol proteins in a cytosolic compartment. These polyproteins more The production of an infectious retrovirus is thus not simply a matter of putting together a multitude of proteins and nucleic acids. Rather, it is a complex progression of many molecular interactions and rearrangements involving an unknown number of intermediate structures.

This chapter summarizes what is currently known about the dynamic process of retroviral assembly. In a sense, it also describes the initial steps of virion disassembly , which can be thought to begin as soon as the assembly of polyproteins and RNA is completed; i. Further uncoating events occur when the virion enters the next cell, as described in Chapter 4. Turn recording back on. National Center for Biotechnology Information , U.

Search term. Figure 1 Model of a typical retroviral particle. Figure 2 Overview of retroviral assembly showing the universal features. Synthesis, Assembly, and Processing of Viral Proteins. Similar articles in PubMed. Review HIV-1 replication. Freed EO. Somat Cell Mol Genet. Assembly and processing of avian retroviral gag polyproteins containing linked protease dimers. J Virol. Characterization of the human endogenous retrovirus K Gag protein: identification of protease cleavage sites.

Epub Mar This may not be the complete list of references from this article. National Center for Biotechnology Information , U. Journal List J Virol v. J Virol. Author information Copyright and License information Disclaimer. Institute of Biotechnology, University of Helsinki, Finland. Copyright notice. This article has been cited by other articles in PMC. Abstract A Semliki Forest virus nonstructural polyprotein, P, expressed in the yeast Saccharomyces cerevisiae in the absence of a replicative RNA template appeared to be properly cleaved into nsP1 to nsP4.

Demonstration in vitro of temperature-sensitive elongation of RNA in Sindbis virus mutant ts6. Solubilization and immunoprecipitation of alphavirus replication complexes. Alphavirus RNA replicase is located on the cytoplasmic surface of endosomes and lysosomes. J Cell Biol. J Gen Virol. Transformation of intact yeast cells treated with alkali cations. J Bacteriol. Selective retention of secretory proteins in the yeast endoplasmic reticulum by treatment of cells with a reducing agent.

Replication of the genome of alphaviruses. J Cell Sci Suppl. Expression of Semliki Forest virus nsP1-specific methyltransferase in insect cells and in Escherichia coli.

A new generation of animal cell expression vectors based on the Semliki Forest virus replicon.